Pierce™ Green Renilla Luc Vector for Luciferase Assays, Expression Vectors, Thermo Scientific

Supplier: Invitrogen

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Supplier: Invitrogen

About this item

Green Renilla Luc vectors contain a gene cloned from the sea pansy, Renilla reniformis

Intracellular Green Renilla luciferase gene, optimised for high expression in mammalian systems
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimise background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells respectively
High-copy pUC bacterial DNA replication origin

pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.
pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the CMV (Cytomegalovirus) promoter. Strong (CMV) constitutive promoters for co-transfection and normalization.
pTK-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter. Weak (TK) constitutive promoters for co-transfection and normalization.

The gene encodes a bioluminescent Green Renilla luciferase (36 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Caution: For Research Use Only. Not for use in diagnostic procedures.

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