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Specifications
- Description:λDE3 Lysogenization Kit
- Environmentally Preferable:
- Cat. No.:EM69734-3
- No. of reactions:10
- Supplier no.:69734-3
Specifications
About this item
The λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E
- Such that the lysogenized host can be used to express target genes cloned in pET vectors
- λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control
- Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant)
T7 Tester Phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG. The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.
coli host cell chromosome.