Millonig's Phosphate Buffers, Electron Microscopy Sciences

About this item

Millonig recommended to use this buffers for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended.

Ingredients: Sodium phosphate, monobasic (NaH₂PO₄.xH₂O), sodium phosphate, dibasic (Na₂HPO₄.xH₂O), 0.5% sodium chloride (NaCl)

The pH of this buffers are 7.4 (0.1 M) and the osmolarity is 440 mosmols, so this buffers are hypertonic to most body fluids.

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