About this item
UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.
- 4X concentrated master mix allows greater flexibility with diluted samples (as little as 1 pg of total RNA) and multiplexing up to 5 targets
- Tough-tested for applications where PCR inhibition from template impurities may be a concern
- Temperature stabilized master mix enables convenient reaction setup at room temperature
UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:
4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).
The system has been optimized to deliver maximum RT‑qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
Caution: UltraPlex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
9 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.
- 4X concentrated master mix allows greater flexibility with diluted samples (as little as 1 pg of total RNA) and multiplexing up to 5 targets
- Tough-tested for applications where PCR inhibition from template impurities may be a concern
- Temperature stabilized master mix enables convenient reaction setup at room temperature
UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:
4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).
The system has been optimized to deliver maximum RT‑qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
Caution: UltraPlex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 10804-944
- 10804-946
- 76121-382
- 76518-322
- 10804-948
- 10804-950
- 10804-952
- 10804-954
- 76470-194
- 76518-324
- Application:
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- RT-qPCR probe
- One step:
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Multiplexing:
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Inhibitor-rich sample:
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
- Yes
Specifications
Product Family Options
Product Information
- Cycler compatibilityNo. of reactionsSizeAvailabilityPrice
- 10804-944UltraPlex 1-Step ToughMixUltraPlex 1-Step ToughMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480100100 x 20 µL rxns (1 x 500 µL)Log in for availabilityEach$297.23Supplier Number: 95166-100Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10804-944ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 10804-946UltraPlex 1-Step ToughMixUltraPlex 1-Step ToughMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480500500 x 20 µL rxns (5 x 500 µL)Log in for availabilityEach$1,218.36Supplier Number: 95166-500Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10804-946ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 76121-382UltraPlex 1-Step ToughMixUltraPlex 1-Step ToughMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 48010001000 x 20 µL rxns (1 x 5 mL)Log in for availabilityKit of 1,000$2,414.68Supplier Number: 95166-01KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.76121-382ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 76518-322UltraPlex 1-Step ToughMixUltraPlex 1-Step ToughMixAgilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 4801000010000 x 20 µL rxns (1 x 50 mL)Log in for availabilityEach$17,982.80Supplier Number: 95166-10KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.76518-322ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 10804-948UltraPlex 1-Step ToughMix ROXUltraPlex 1-Step ToughMix ROXABI 7900HT/7900HT Fast/StepOnePlus™100100 x 20 µL rxns (1 x 500 µL)Log in for availabilityEach$213.76Supplier Number: 95167-100Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
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Specifications
Cat. No.10804-948ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - UltraPlex 1-Step ToughMix ROXABI 7900HT/7900HT Fast/StepOnePlus™500500 x 20 µL rxns (5 x 500 µL)Discontinued-Supplier Number: -Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10804-950ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - UltraPlex 1-Step ToughMix Low ROXABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx100100 x 20 µL rxns (1 x 500 µL)Log in for availabilityEach$296.22Supplier Number: 95168-100Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10804-952ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 10804-954UltraPlex 1-Step ToughMix Low ROXUltraPlex 1-Step ToughMix Low ROXABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx500500 x 20 µL rxns (5 x 500 µL)Log in for availabilityEach$1,240.50Supplier Number: 95168-500Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10804-954ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 76470-194UltraPlex 1-Step ToughMix Low ROXUltraPlex 1-Step ToughMix Low ROXABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx10001000 x 20 µL rxns (1 x 5 mL)Log in for availabilityEach$1,905.37Supplier Number: 95168-01KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.76470-194ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes - 76518-324UltraPlex 1-Step ToughMix Low ROXUltraPlex 1-Step ToughMix Low ROXABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx1000010000 x 20 µL rxns (1 x 50 mL)Log in for availabilityEach$17,982.80Supplier Number: 95168-10KItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.76518-324ApplicationRT-qPCR probeOne stepYesMultiplexingYesInhibitor-rich sampleYes
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