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UltraPlex 1-Step ToughMix (4X), QuantaBio

UltraPlex 1-Step ToughMix (4X), QuantaBio

UltraPlex 1-Step ToughMix (4X), QuantaBio
Supplier:  Quantabio
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UltraPlex 1-Step ToughMix (4X), QuantaBio

9 Options Available Below

  • Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
  • Return Policy
Product Details & Documents

UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.

  • 4X concentrated master mix allows greater flexibility with diluted samples (as little as 1 pg of total RNA) and multiplexing up to 5 targets
  • Tough-tested for applications where PCR inhibition from template impurities may be a concern
  • Temperature stabilized master mix enables convenient reaction setup at room temperature

UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:
4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).

The system has been optimized to deliver maximum RT‑qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Caution: UltraPlex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.

Specifications for Product Family

Specifications

Specifications

  • Catalog No:
  • 10804-944
  • 10804-946
  • 76121-382
  • 76518-322
  • 10804-948
  • 10804-950
  • 10804-952
  • 10804-954
  • 76470-194
  • 76518-324
  • Application:
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • RT-qPCR probe
  • One step:
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Multiplexing:
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Inhibitor-rich sample:
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes
  • Yes

Specifications

Product Family Options

Product Information

  • Cycler compatibility
    No. of reactions
    Size
    Availability
    Price
  • 10804-944
    UltraPlex 1-Step ToughMix
    UltraPlex 1-Step ToughMix
    Agilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480
    100
    100 x 20 µL rxns (1 x 500 µL)
    Log in for availability
    Log in for availability
    Each
    Each - $297.23
    $297.23
    Each
       $297.23
    Minimum order quantity is 1

    Supplier Number: 95166-100
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-944
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 10804-946
    UltraPlex 1-Step ToughMix
    UltraPlex 1-Step ToughMix
    Agilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480
    500
    500 x 20 µL rxns (5 x 500 µL)
    Log in for availability
    Log in for availability
    Each
    Each - $1,218.36
    $1,218.36
    Each
       $1,218.36

    Supplier Number: 95166-500
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-946
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 76121-382
    UltraPlex 1-Step ToughMix
    UltraPlex 1-Step ToughMix
    Agilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480
    1000
    1000 x 20 µL rxns (1 x 5 mL)
    Log in for availability
    Log in for availability
    Kit of 1,000
    Kit of 1,000 - $2,414.68
    $2,414.68
    Kit of 1,000
       $2,414.68
    Minimum order quantity is 1

    Supplier Number: 95166-01K
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    76121-382
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 76518-322
    UltraPlex 1-Step ToughMix
    UltraPlex 1-Step ToughMix
    Agilent AriaMx, BioRad CFX, QIAGEN Rotor-Gene Q, Quantabio Q, Roche LightCycler 480
    10000
    10000 x 20 µL rxns (1 x 50 mL)
    Log in for availability
    Log in for availability
    Each
    Each - $17,982.80
    $17,982.80
    Each
       $17,982.80
    Minimum order quantity is 1

    Supplier Number: 95166-10K
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    76518-322
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 10804-948
    UltraPlex 1-Step ToughMix ROX
    UltraPlex 1-Step ToughMix ROX
    ABI 7900HT/7900HT Fast/StepOnePlus™
    100
    100 x 20 µL rxns (1 x 500 µL)
    Log in for availability
    Log in for availability
    Each
    Each - $213.76
    $213.76
    Each
       $213.76
    Minimum order quantity is 1

    Supplier Number: 95167-100
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-948
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 10804-950
    UltraPlex 1-Step ToughMix ROX
    UltraPlex 1-Step ToughMix ROX
    ABI 7900HT/7900HT Fast/StepOnePlus™
    500
    500 x 20 µL rxns (5 x 500 µL)
    Discontinued
    Supplier Number: -
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-950
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 10804-952
    UltraPlex 1-Step ToughMix Low ROX
    UltraPlex 1-Step ToughMix Low ROX
    ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx
    100
    100 x 20 µL rxns (1 x 500 µL)
    Log in for availability
    Log in for availability
    Each
    Each - $296.22
    $296.22
    Each
       $296.22
    Minimum order quantity is 1

    Supplier Number: 95168-100
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-952
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 10804-954
    UltraPlex 1-Step ToughMix Low ROX
    UltraPlex 1-Step ToughMix Low ROX
    ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx
    500
    500 x 20 µL rxns (5 x 500 µL)
    Log in for availability
    Log in for availability
    Each
    Each - $1,240.50
    $1,240.50
    Each
       $1,240.50

    Supplier Number: 95168-500
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    10804-954
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 76470-194
    UltraPlex 1-Step ToughMix Low ROX
    UltraPlex 1-Step ToughMix Low ROX
    ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx
    1000
    1000 x 20 µL rxns (1 x 5 mL)
    Log in for availability
    Log in for availability
    Each
    Each - $1,905.37
    $1,905.37
    Each
       $1,905.37

    Supplier Number: 95168-01K
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    76470-194
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
  • 76518-324
    UltraPlex 1-Step ToughMix Low ROX
    UltraPlex 1-Step ToughMix Low ROX
    ABI 7500/7500 Fast/QuantStudio™, Agilent AriaMx, Douglas Scientific IntelliQube®, Stratagene Mx
    10000
    10000 x 20 µL rxns (1 x 50 mL)
    Log in for availability
    Log in for availability
    Each
    Each - $17,982.80
    $17,982.80
    Each
       $17,982.80
    Minimum order quantity is 1

    Supplier Number: 95168-10K
    Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
    Specifications:
    More
    Specifications
    Cat. No.
    76518-324
    Application
    RT-qPCR probe
    One step
    Yes
    Multiplexing
    Yes
    Inhibitor-rich sample
    Yes
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