Human Recombinant BDNF (from E. coli)

About this item

BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant Human BDNF is a 27.0 kDa homodimer of two 120 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical. Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Verified by N-terminal and Mass Spectrometry analyses (when applicable).

Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.

Specifications

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological Activity:
Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED₅₀ for this effect is 0.5-1.0 μg/ml.
Animal-Free:
Y
Protein/Peptide Name:
BDNF
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping Temperature:
RT