Human Recombinant IFNG (from E. coli)

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant Human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues. Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Verified by N-terminal and Mass Spectrometry analyses (when applicable).

: For research use only. Not for use in diagnostic or therapeutic procedures.

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological Activity:
Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED₅₀ for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED₅₀ was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
Animal-Free:
Y
Protein/Peptide Name:
IFNG
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping Temperature:
RT