Human Recombinant IL4 (from E. coli)

About this item

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant Human IL-4 is a 15.1 kDa globular protein containing 130 amino acid residues. Manufactured using all Animal-Free reagents.

Carrier free
Animal-free

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Verified by N-terminal and Mass Spectrometry analyses (when applicable).

Ordering information: For research use only. Not for use in diagnostic or therapeutic procedures.

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Specifications

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
−20 °C
Endotoxin Content:
<0.1 ng/μg of protein (<1 EU/μg).
Biological Activity:
The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁶ units/mg.
Animal-Free:
Y
Protein/Peptide Name:
IL4
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Formulation:
Lyophilized
Shipping Temperature:
RT