- Antibody Type:Primary
- Antigen Name:Cyclin-dependent Kinase 2
- Antigen Symbol:CDK2
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Western Blot:Yes
- Size:100 µL
- Cross Adsorption:No
- Form:liquid
- Gene ID:1017
- Antigen Synonyms:Cell division protein kinase 2|CDH2 T160,Cyclin-dependent kinase 2|p-CDH2 Thr160|CDK2_HUMAN.|p33 protein kinase|CDH2 Thr160
- Storage Buffer:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
- Storage Temperature:Store at –20 °C for 12 months
- Concentration:1 μg/μl
- Shipping Temperature:4°C
- Immunogen:220-260/298
- Purification:Purified by Protein A
- Cat. No.:10245-192
- Supplier no.:BS-10726R
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication.
Recommended Dilutions: Western Blot: 1:100-1000
Type: Primary
Antigen: CDK2
Clonality: Polyclonal
Clone: 0
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: