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Specifications
- Application:
- Description:Classic++™ 1-Step RT-PCR Kit
- Environmentally Preferable:
- Cat. No.:10002-274
- No. of reactions:50
- Supplier no.:31-5220-0050R
Specifications
About this item
The Classic++ 1-Step RT-PCR kit’s advanced reagents allow for efficient cDNA synthesis and PCR in a single tube
For the PCR reaction, Classic++ Hot Start Taq DNA Polymerase is included. Novel hot start technology improves yields by minimizing or eliminating primer dimer formation and non-specific amplification.
Convenient RT-PCR kit formulation allows for high speed, accurate and reproducible cDNA synthesis and subsequent PCR amplification in a single tube, starting from a variety of target RNA templates.
The kit contains a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, a mix that is both thermostable and extremely active. This RTase mix synthesizes cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.