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- Antibody Type:Primary
- Antigen Symbol:NOP1
- Clonality:Monoclonal
- Clone:38F3
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoFluorescence:Yes
- Reactivity:Yeast,Human,Rat
- Western Blot:Yes
- Size:100 µL
- Format:IgG1
- Form:Liquid
- Gene ID:851548
- Concentration:Titrated value sufficient to run approximately 10 mini blots.
- Shipping Temperature:Dry Ice
- Immunogen:Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.
- Purification:Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are a total IgG fraction from concentrated clarified cell culture supernate. Reactivity is expected against human and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
- Cat. No.:10802-810
- Supplier no.:900-301-D39
Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.
Recommended Dilutions: Immunofluorescence Microscopy: 1:500; Western Blot: 1:1000; contains 0.01% (w/v) Sodium Azide
Type: Primary
Antigen: NOP1
Clonality: Monoclonal
Clone: 38F3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Yeast, Human, Rat