Specifications
- Antibody Type:Tag
- Antigen Name:6X HIS Affinity Tag
- Antigen Symbol:6xHis
- Clonality:Monoclonal
- Clone:33D10.D2.G8
- Conjugation:Cy3®
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoFluorescence:Yes
- Size:50 µg
- Format:IgG1
- Form:Lyophilized
- Antigen Synonyms:Histidine Epitope Tag|His Tag|Histidine Tag
- Concentration:1.0 mg/mL by UV absorbance at 280 nm
- Shipping Temperature:Ambient
- Immunogen:HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
- Tested Applications:Fluoresence ELISA, Fluorochrome Protein
- Purification:6X HIS Epitope Tag CY3 conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
- Cat. No.:10799-508
- Supplier no.:200-304-382
Specifications
About this item
6X His Tag CY3 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Recommended Dilutions: FLISA: 1:10,000 - 1:50,000; Flow Cytometry: 1:500 - 1:2,500; Fluorochrome Protein Value: 8.1; Immunofluorescence Microscopy: 1:1,000 - 1:5,000 ; contains 0.01% (w/v) Sodium Azide
Type: Tag
Antigen: 6xHis
Clonality: Monoclonal
Clone: 33D1.D2.G8
Conjugation: Cy3®
Epitope:
Host: Mouse
Isotype:
Reactivity: