Anti-I-A/I-E Rat Monoclonal Antibody (APC (Allophycocyanin)) [clone: M5/114.15.2]

About this item

APC anti-mouse I-A/I-E [M5/114.15.2]; Isotype: Rat IgG2b, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining,the suggested use of this reagent is 0.25 μg per 10⁶cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Type: Primary
Antigen: I-A/I-E
Clonality: Monoclonal
Clone: M5/114.15.2
Conjugation: APC (Allophycocyanin)
Epitope:
Host: Rat
Isotype: Rat IgG2b, κ
Reactivity: Mouse

Specifications

Catalog No:
107613-BL
107614-BL

Antigen Symbol:
I-A/I-E
I-A/I-E

Antigen Synonyms:
MHC Class II
MHC Class II

Antibody Type:
Primary
Primary

Clonality:
Monoclonal
Monoclonal

Conjugation:
APC (Allophycocyanin)
APC (Allophycocyanin)

Clone:
M5/114.15.2
M5/114.15.2

Reactivity:
Mouse
Mouse

Host:
Rat
Rat

Gene ID:
14961, 14969
14961, 14969

Isotype:
IgG2b kappa
IgG2b kappa

Flow Cytometry:
Yes
Yes

Immunogen:
Activated C57BL/6 mouse spleen cells
Activated C57BL/6 mouse spleen cells

Form:
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Purification:
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Concentration:
0.20 mg/mL
0.20 mg/mL

Storage Temperature:
The antibody solution should be stored undiluted between 2 °C and 8 °C, and protected from prolonged exposure to light. Do not freeze.
The antibody solution should be stored undiluted between 2 °C and 8 °C, and protected from prolonged exposure to light. Do not freeze.