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Specifications
- Size:100 assays
- Environmentally Preferable:
- Species Reactivity:Mammalian samples
- Shelf Life:12 Months
- Sample Size:Serum, plasma, urine or other biological fluids and Mammalian tissues: kidney, liver, brain samples etc.
- Storage Temperature:-20°C
- Cat. No.:10191-118
- Supplier no.:K556-100
Specifications
About this item
Detection method- Absorbance (450 nm)
Applications- Measurement of Glutamine in various biological samples
Glutamine (Gln) is one of the most abundant amino acids containing an uncharged amide as a side chain. It is synthesized via condensation of glutamate and ammonia. Gln is classified as a non-essential amino acid, however, Gln is important in several biological processes such as protein synthesis, regulation of acid-balance in mammalian kidneys and cell growth. It constitutes cell’s main source of nitrogen for the synthesis of nucleotides and hexosamines. Glutamine-rich diets benefit patients suffering from Crohn’s disease, severe burns, HIV/AIDS and cancer. BioVision’s Glutamine Colorimetric Assay kit is a simple and sensitive assay that detects the biologically relevant concentrations of Gln in various fluids and tissues. The assay is based on the hydrolysis of Glutamine to Glutamate producing a stable signal, which is directly proportional to the amount of Gln in the sample. The assay can detect as little as 25 µM of Gln in a variety of biological samples.