Order Entry
United States
ContactUsLinkComponent
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit, BioVision
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit, BioVision
  10186-832
 :  Avantor
 :  
undefined
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit, BioVision
  10186-832
 :  Avantor
 :  
Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

  • • State issued document with your organization's Federal Tax ID Number
  • • State issued document with your organization's Resale Tax ID Number
  • • City or County issued Business License
  • • State Department of Health Services License
  • • Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.

 

  • Size:
    100 assays
  • Environmentally Preferable:
  • Species Reactivity:
    Mammalian samples
  • Shelf Life:
    12 Months
  • Sample Size:
    Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids
  • Storage Temperature:
    -20°C
  • Cat. No.:
    10186-832
  • Supplier no.:
    K564-100

 

 

Detection method- Absorbance (450 nm)
Species reactivity- Mammalian
Applications- BioVision’s BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample).

The branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects. BioVision's BCAA Assay Kit provides a simple convenient means of measuring the BCAA’s in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). BioVision’s BCAA kit measures BCAA’s in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA’s are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).