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Specifications
- Size:60 assays
- Species Reactivity:Mammalian samples
- Shelf Life:12 Months
- Sample Size:Cell cultures (adherent and suspension), parafin embedded tissue sections
- Storage Temperature:-20°C
- Cat. No.:10186-684
- Supplier no.:K401-60
Specifications
About this item
Detects DNA Fragmentation by FACS or FL
Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)
Species reactivity- Mammalian
Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.