Caspase-3 Colorimetric Assay Kit, BioVision

Supplier: Avantor

Supplier: Avantor

About this item

Convenient, Highly-Sensitive Assay

Detection method- Absorbance (400 or 405 nm)
Species reactivity- Mammalian
Kit size- Convenient sizes (25, 100, 200, 400 assays)
Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

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