The Overnight Express™ Autoinduction System 1 enables high-level protein expression without the need to monitor cell growth or add an inducer during the growth period.

• Autoinduction System is extremely convenient for routine expression of proteins in multiple cultures
• Minimal sample handling
• Convenient for high-throughput production of proteins for structural or functional analysis
• Increased soluble protein yield
• Induction of numerous expression clones simultaneously
• Compatible with cultures grown in flasks, culture tubes, and deep-well plates
• Ideal for high-throughput parallel analysis of protein expression, solubility and purification from multiple expression clones

The kit contains three sterile components: OnEx™ Solution 1: A blend of carbon sources optimized for tightly regulated uninduced growth to high cell density, followed by high-level induction

With Overnight Express™ Autoinduction System 1, a period of cell growth is followed by spontaneous induction of protein expression–without monitoring cell density and without conventional induction with IPTG. The method is based on media components that are metabolized differentially to promote growth to high density and automatically induce protein expression from lac promoters.

OnEx™ Solution 2: A concentrated buffer and nitrogen-source blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis. OnEx™ Solution 3: Provides magnesium for maximal cell density.