Specifications
- Antibody Type:Primary
- Antigen Name:Neurofilament, Heavy chain
- Antigen Symbol:NHC
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Rat
- Western Blot:Yes
- Size:50 µL
- Epitope:Multiple points of reactivity likely because of the polyclonal nature of the antibody
- Cross Adsorption:No
- Format:Lyophilized
- Antigen Synonyms:Neurofilament triplet H protein|KIAA0845|NF-H|NHC|NFH|Neurofilament heavy polypeptide|200 kDa neurofilament protein
- Immunogen:Purified rat NF-H construct containing most of the tandem KSP repeats expressed and purified from E.coli.
- Cat. No.:10782-462
- Supplier no.:R-1389-50
Specifications
About this item
Neurofilaments contain three intermediate filament proteins: light (68 kDa), medium (160 kDa) and heavy (200 kDa). Neurofilament heavy (NF200 or NF-H) is phosphorylated and it is thought that this results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
The specificity of this antibody has been confirmed by WB. This antibody reacts with noth phosphorylated NF-H at approx 200 kDa and non-phosphorylated NF-H at 160 kDa.
Application Information:
Western Blotting (WB) and Immunocytochemistry (IC). Suggested dilution for WB of 1:5,000-10,000. Suggested dilution for IC is 1:500-1,000. This antibody stains dendritic and perikaryal neurofilaments particularly well. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: Neurofilament Heavy, phosphorylated and non-phosphorylated
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Multiple points of reactivity likely because of the polyclonal nature of the antibody
Host: Rabbit
Isotype: IgG
Reactivity: Rat