The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. Yields up to 15ug of plasmid DNA from 600ul to 3ml of bacterial culture.

• Up to 15microg of Transfection-Ready Plasmid from 3ml Cultures
• Rapid, 10-minute protocol
• Highly pure, concentrated plasmid DNA
• No tedious, gravity-drip columns or post-elution alcohol precipitation

The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15ug of plasmid DNA from 600ul to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TNT Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method. The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man Laboratory Vacuum Manifold).

Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in-vitro transcription, and coupled in-vitro transcription and translation (e.g., TnT® Quick Coupled Transcription and Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.

The PureYield Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA, and endotoxin contaminants from purified plasmid DNA.