SNAP-Cell 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro

• Elute in as little as 30 μl
• Eliminate buffer retention and salt carryover with optimized column design
• Save time with fast, user-friendly protocols
• Easily label columns using tab and frosted surfaces
• Monitor completion of certain steps and easily identify buffers with colored buffer system
• No need to add RNase before starting
• Reduced environmental impact
• Recyclable packaging

This cell-permeable substrate (also termed SiR-SNAP) is based on 6-carboxy-tetramethylsiliconrhodamine and is suitable for standard Cy5 filter sets. It has an excitation maximum at 645 nm and an emission maximum at 661 nm. This package contains 30 nmol of SNAP-Cell 647-SiR substrate, sufficient to make 10 ml of a 3 μM SNAP-tag fusion protein labeling solution.