Trypsin/ EDTA
About this item
Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
- With EDTA
- With Phenol Red
Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent often added to enhance enzymatic activity of trypsin solution. EDTA acts by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion. Polyvinylpyrrolidone (PVP) a synthetic polymer is known to increase cell viability especially in low serum or serum free condition.
Sterile Filtered
Performance Tested
Certifications: Manufactured in an ISO 9001:2015 certified facility.
Ordering information: *NOTE: During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not effect the functionality of the trypsin.
Caution: Research Use Only
4 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
- With EDTA
- With Phenol Red
Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carboxyl sides of Lysine and Arginine, except when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent often added to enhance enzymatic activity of trypsin solution. EDTA acts by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion. Polyvinylpyrrolidone (PVP) a synthetic polymer is known to increase cell viability especially in low serum or serum free condition.
Sterile Filtered
Performance Tested
Certifications: Manufactured in an ISO 9001:2015 certified facility.
Ordering information: *NOTE: During shipping, the trypsin might change color from pink to yellow due to exposure to carbon dioxide released from the dry ice in the shipping container. In our experience, this discoloration does not effect the functionality of the trypsin.
Caution: Research Use Only
Documents
Certificates
Search by Lot NumberSpecifications for Product Family
Specifications
Specifications
- Catalog No:
- 10128-786
- 10128-784
- 10128-794
- 10128-792
- Shipping Conditions:
- on dry ice
- on dry ice
- on dry ice
- on dry ice
- Storage Conditions:
- –10 °C…–20 °C
- –10 °C…–20 °C
- –10 °C…–20 °C
- –10 °C…–20 °C
Specifications
Product Family Options
Product Information
- FormulationSizeAvailabilityPrice
- 10128-786Trypsin (0.05%), EDTA (0.1%)Trypsin (0.05%), EDTA (0.1%)Membrane Filtered; Prepared with Dulbecco's-PBS without calcium and magnesium. Contains 0.0005% (w/v) phenol red100 mlLog in for availabilityEach (100ml)$28.45Supplier Number: 118-087-721EAItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Minority or Woman-Owned Business EnterpriseSmall Business Enterprise
More
Specifications
Cat. No.10128-786Shipping Conditionson dry iceStorage Conditions–10 °C…–20 °CMinority or Woman-Owned Business EnterpriseSmall Business Enterprise - 10128-784Trypsin (0.05%), EDTA (0.1%)Trypsin (0.05%), EDTA (0.1%)Sterile filtered, contains Phenol Red4x100 mlLog in for availabilityPack of 4 (400ml)$96.75Supplier Number: 118-087-721Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Minority or Woman-Owned Business EnterpriseSmall Business Enterprise
More
Specifications
Cat. No.10128-784Shipping Conditionson dry iceStorage Conditions–10 °C…–20 °CMinority or Woman-Owned Business EnterpriseSmall Business Enterprise - 10128-794Trypsin (0.25%), EDTA (0.02%)Trypsin (0.25%), EDTA (0.02%)Membrane Filtered; prepared with Hanks' Balanced Salt Solution (HBSS) without calcium and magnesium100 mLLog in for availabilityEach (100ml)$49.27Supplier Number: 118-093-721EAItem requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Minority or Woman-Owned Business EnterpriseSmall Business Enterprise
More
Specifications
Cat. No.10128-794Shipping Conditionson dry iceStorage Conditions–10 °C…–20 °CMinority or Woman-Owned Business EnterpriseSmall Business Enterprise - 10128-792Trypsin (0.25%), EDTA (0.02%)Trypsin (0.25%), EDTA (0.02%)Sterile filtered, contains Phenol Red4x100 mlLog in for availabilityPack of 4 (100ml)$165.10Supplier Number: 118-093-721Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:Minority or Woman-Owned Business EnterpriseSmall Business Enterprise
More
Specifications
Cat. No.10128-792Shipping Conditionson dry iceStorage Conditions–10 °C…–20 °C
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