About this item
Precision Plus Protein™ Unstained Standards contain ten highly purified recombinant proteins with molecular masses from 10 to 250 kD, of which three are high-intensity reference bands (25, 50, and 75 kD)
These standards offer absolute molecular weight accuracy confirmed by mass spectrometry, with a known amount of protein in each band to allow approximation of protein concentration in your sample. All Precision Plus Protein™ Standards generate a standard curve with r2 values>0.99, demonstrating a strong linear relationship between the proteins' log molecular weight and relative migration distance on a gel.
Precision Plus Protein™ Unstained Standards are compatible with Laemmli and neutral pH buffer gel systems. These unstained standards can be used with stain-free gels and the Bio-Rad stain-free enabled imaging systems.
These standards have been engineered to contain a Strep-tag affinity peptide, which allows detection and molecular weight determination on western blots.
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Specifications
- Loading Volume:10 µL(for Coomassie and Stain-Free Gels);1 to 3 µL(for Silver Staining and SYPRO Ruby Staining);1 to 10 µL(for Blot Development)
- Shelf Life:12 months at –20°C
- Dilution Factor:1:5,000 to 1:15,000
- Molecular Weight:10–250 kD
- Prestained Protein Markers:Blue bands; 635 nm laser or red LED: pink bands; 532 nm laser or green LED
- Application:Electrophoretic Separation and Blot Transfer