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Specifications
- Size:1 kit
- Cat. No.:IC15883701
- Supplier no.:15883701
Specifications
About this item
Typical working concentration for 100 mL of inhibitor cocktail: AEBSF Hydrochloride:50-400 mg/mL; EDTA disodium dihydrate:0.2-25 mg/mL; Leupeptin Hemisulfate: 0.5-1 mg/mL; Pepstatin A: 0.5-1 mg/mL
Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles.
Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstain A.
Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.
Proteases are enzymes which direct their action upon proteins, cleaving their targets into smaller fragments consisting of polypeptides, peptides and amino acids. There are a myriad of proteases active against practically all proteins. Some proteases are quite specific, while others cleave many different proteins. MP Protease Inhibitor Cocktail covers a broad spectrum of protese inhibitors.