To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Source:
- Species:
- Size:1 kit
- Environmentally Preferable:
- Cat. No.:IC15883701
- Supplier no.:15883701
Typical working concentration for 100 mL of inhibitor cocktail: AEBSF Hydrochloride:50-400 mg/mL; EDTA disodium dihydrate:0.2-25 mg/mL; Leupeptin Hemisulfate: 0.5-1 mg/mL; Pepstatin A: 0.5-1 mg/mL
Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles.
Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstain A.
Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.
Proteases are enzymes which direct their action upon proteins, cleaving their targets into smaller fragments consisting of polypeptides, peptides and amino acids. There are a myriad of proteases active against practically all proteins. Some proteases are quite specific, while others cleave many different proteins. MP Protease Inhibitor Cocktail covers a broad spectrum of protese inhibitors.