Bovine Carbonic Anhydrase (from Erythrocytes), MP Biomedicals

Soluble in distilled water or dilute buffer.

The Unit is determined by the electrometic method in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCI buffer from 8.3 to 6.3 at 0 °C is determined. The time without enzyme is recorded at To; with enzyme T. One unit equals 2X (To-T)/T using the electrometric assay.

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