Excess cholesterol is stored intracellularly as cholesterol esters.

• Presentation: Yellowish tan lyophilized powder
• Isoelectric point (pl): 5.9 ±0.1(Lit.)
• Optimum pH: 7.0 to 9.0 ; pH stability: pH 5.0 to 9.0 (25 °C, 24 h)
• Soluble in distilled water or dilute buffer

Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols.