The Thermo Scientific™ GlycanPac™ AXR-1 column is a high-performance, silica-based HPLC column for the simultaneous separation of glycans by charge, isomerism, and size. It is designed to provide industry-leading resolution with unique selectivities for biologically important glycans, either labeled or native, using either fluorescence and/or mass spectrometry (MS) detection.

• Unique glycan selectivity based on charge, branch/linkage isomerism, and size
• Exceptional resolution for either native or derivatized glycans
• Compatibility with both MS and fluorescence detection methods

The GlycanPac AXR-1 column combines both weak anion-exchange (WAX) and reversed-phase interaction mechanisms to produce exceptional glycan selectivity and resolution. The WAX functionality provides retention of negatively charged glycans that elute in order of increasing charge, and the reversed-phase mode facilitates resolution of glycans of the same charge according to their isomerism and size. The GlycanPac AXR-1 column is designed for HPLC and UHPLC methods using either fluorescence or MS detection, and uses volatile aqueous buffers (e.g., ammonium acetate or ammonium formate) and acetonitrile, presenting the eluting glycans ready for introduction into MS instruments.