Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Western Blot: Multi-Cells/Tissue, 1:5000-1:20000; IHC: Human breast cancer Tissue, 1:20-1:200; IP:N/A, 1:2000-1:20000; IF:Ethacrynic acid treated HeLa Cells, 1:20-1:200; FC: HeLa Cells, N/A

Type: Primary
Antigen: GAPDH
Clonality: Monoclonal
Clone: 1E6D9
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human, Mouse, Rat, Zebrafish