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- Antibody Type:Primary
- Antigen Name:Ring Finger And WD Repeat Domain 3
- Antigen Symbol:RFWD3
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western Blot:Yes
- Size:150 µL
- Antigen Synonyms:RNF201
- Storage Buffer:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
- Shipping Temperature:Store at -20°C
- Immunogen:Fusion Protein
- Purification:Antigen affinity Purification
- Cat. No.:10093-602
- Supplier no.:19893-1-AP
RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.
Western Blot: Mouse lung Tissue, 1:500-1:5000; IHC: Human Testis, 1:20-1:200; IF: Hela, 1:20-1:200
Type: Primary
Antigen: RFWD3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat