Order Entry
United States
ContactUsLinkComponent
Human Recombinant WNT7A (from HEK293 Cells)
Human Recombinant WNT7A (from HEK293 Cells)
Catalog # 10073-060
Supplier:  Prosci
CAS Number:  
Human Recombinant WNT7A (from HEK293 Cells)
Catalog # 10073-060
Supplier:  Prosci
Supplier Number:  40-577
CAS Number:  

Specifications

  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    Human
  • Size:
    0.003 mg
  • Biological Activity:
    Biological activity was determined by its ability to inhibit Wnt3a induced alkaline phosphatase production in MC3T3-E1 cells. The expected ED50 for this effect is 40-60 ng/mL. Endotoxin level is less than 0.1 ng per μg of WNT-7A (1EU/μg).
  • Protein Synonyms:
    Protein Wnt-7a
  • Protein/Peptide Name:
    WNT7A
  • Purity:
    >80%
  • Formulation:
    Lyophilized
  • Cat. No.:
    10073-060
  • Supplier no.:
    40-577

Specifications

About this item

Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. It is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions.

Store in working aliquots at -20°C. Avoid freeze/thaw cycles.