Thymus Lysate, ProSci
About this item
Thymus tissue lysate (Human) was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors)
Thymus tissue lysate (Mouse and Rat) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
3 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Thymus tissue lysate (Human) was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors)
Thymus tissue lysate (Mouse and Rat) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 10062-224
- 10062-268
- 10062-332
- Concentration:
- 2 mg/mL
- 2 mg/mL
- 2 mg/mL
- Tested Applications:
- WB
- WB
- WB
- Storage Buffer:
- Thymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Thymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Thymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Storage Conditions:
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
- Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
Specifications
Product Family Options
Product Information
- Cell FormatSizeSourceTissueAvailabilityPrice
- 10062-224NormalSupplier Number: 1409Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10062-224Concentration2 mg/mLTested ApplicationsWBStorage BufferThymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage ConditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. - 10062-268NormalSupplier Number: 1471Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10062-268Concentration2 mg/mLTested ApplicationsWBStorage BufferThymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage ConditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C. - 10062-332NormalSupplier Number: 1314Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.10062-332Concentration2 mg/mLTested ApplicationsWBStorage BufferThymus lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.Storage ConditionsStore at 2-8°C for continuous use. For extended storage, freeze working aliquots at -70°C.
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