Platelet factor 4 (PF-4) is a 70 amino acid protein that is released from the alpha granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF-4 probably has a role in inflammation and wound repair.

ELISA: To detect hPF-4 by direct ELISA (using 100 µL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hPF-4. Sandwich: To detect hPF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25 - 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human PF-4 (XP-5256) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ?g/lane, under either reducing or non-reducing conditions.

Anti-hPF-4 specific antibody was purified by affinity chromatography and then biotinylated.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: PF4
Clonality: Polyclonal
Clone:
Conjugation: Biotin
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human