Specifications
- Antibody Type:Primary
- Antigen Name:ret proto-oncogene
- Antigen Symbol:RET
- Clonality:Monoclonal
- Clone:8D10C9
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG1
- Reactivity:Human
- Western Blot:Yes
- Size:0.1 mL
- Gene ID:P07949
- Antigen Synonyms:RET
- Storage Buffer:Ascitic fluid containing 0.03% sodium azide.
- Storage Temperature:RET monoclonal antibody can be stored at –20 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Shipping Temperature:4 °C
- Immunogen:Purified truncated recombinant RET (aa896-1063)-GST fusion protein is expressed in E. Coli strain BL21 (DE3).
- Cat. No.:10070-664
- Supplier no.:32-210
Specifications
About this item
RET (ret proto-oncogene) is a member of the cadherin superfamily and a receptor tyrosine kinase, which are cell-surface molecules that transduce signals for cell growth and differentiation. It can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Alterations in the corresponding Ret gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr905 dramatically inhibits Ret autophosphorylation activity.
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Ascitic fluid containing 0.03% sodium azide.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: RET
Clonality: Monoclonal
Clone: 8D10C9
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human