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Anti-SMAD7 Rabbit Polyclonal Antibody
Anti-SMAD7 Rabbit Polyclonal Antibody
Catalog # 10105-016
Supplier:  Prosci
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Anti-SMAD7 Rabbit Polyclonal Antibody
Catalog # 10105-016
Supplier:  Prosci
Supplier Number:  27-478
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Specifications

  • Antibody Type:
    Primary
  • Antigen Name:
    SMAD family member 7
  • Antigen Symbol:
    SMAD7
  • Clonality:
    Polyclonal
  • Conjugation:
    Unconjugated
  • ELISA:
    Yes
  • Host:
    Rabbit
  • Isotype:
  • Reactivity:
    Human
  • Western Blot:
    Yes
  • Size:
    100 ul
  • Environmentally Preferable:
  • Form:
    Liquid
  • Gene ID:
    O15105
  • Antigen Synonyms:
    SMAD7
  • Storage Buffer:
    Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Molecular Weight:
    46 kDa
  • Storage Temperature:
    For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMAD7 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
  • Shipping Temperature:
    4 °C
  • Immunogen:
    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.
  • Purification:
    Antibody is purified by protein A chromatography method.
  • Cat. No.:
    10105-016
  • Supplier no.:
    27-478

Specifications

About this item

Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.

SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:62500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.25 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is purified by protein A chromatography method.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type: Primary
Antigen: SMAD7
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human