Specifications
- Antibody Type:Primary
- Antigen Name:UPF1 regulator of nonsense transcripts homolog (yeast)
- Antigen Symbol:UPF1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- ELISA:Yes
- Host:Rabbit
- Reactivity:Human
- Western Blot:Yes
- Size:100 ul
- Form:Liquid
- Gene ID:Q92900
- Antigen Synonyms:UPF1
- Storage Buffer:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Molecular Weight:123 kDa
- Storage Temperature:For short periods of storage (days) store at 4 °C. For longer periods of storage, store UPF1 antibody at –20 °C. As with any antibody avoid repeat freeze-thaw cycles.
- Shipping Temperature:4 °C
- Immunogen:Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UPF1.
- Purification:Antibody is purified by peptide affinity chromatography method.
- Cat. No.:10101-888
- Supplier no.:25-352
Specifications
About this item
UPF1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
UPF1 antibody can be used for detection of UPF1 by ELISA at 1:312500. UPF1 antibody can be used for detection of UPF1 by western blot at 1 ?g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody is purified by peptide affinity chromatography method.
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type: Primary
Antigen: UPF1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human