TSKgel SWXL columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

TSKgel SWXL columns contain 5 and 8 μm particles. These columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWXL columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.

Both the TSKgel G2000SWXL and G3000SWXL columns contain 5 μm particles and are excellent choices for small proteins and peptide separations. TSKgel G2000SWXL has 12.5 nm pores with a fractionation range that is applicable for globular protein samples up to 150000 Da. With 25 nm pores, the TSKgel G3000SWXL columns have a fractionation range applicable for globular protein samples up to 0.5 million Da. The TSKgel G4000SWXL columns contain 8 μm particles and 45 nm pores, providing the largest exclusion limit and the widest fractionation range. It is an excellent choice for pegylated proteins or glycosylated biomolecules. The fractionation range is applicable for globular protein samples up to 7 million Da.