The MP ouchterlony plate is designed for specific but low affinity antisera

• Typically dilutions of 1:600 to 1:16,000 are required

Each MP immunodiffusion plate contains 8 ouchterlony patterns consisting of a center well and 6 outer wells.The high quality immunodiffusion grade agarose contains 5% PEG which greatly enhances the sensitivity of the immunodiffusion assay. The plates contain 1.2% agarose (buffer - PBS with 0.1% sodium azide and 0.01% merthiolate). The maximum sample volume is 10 ul in any of the wells.

Such antisera require properly spaced wells and olyethylene glycol in the agarose for optimal immuno -precipitation.