The PURExpress® Disulfide Bond Enhancer (PDBE) is a proprietary blend of proteins and buffer components designed to correctly fold target proteins with multiple disulfide bonds produced in PURExpress reactions or E.coli S30 extracts.

Added at the beginning of a reaction, the components promote a proper disulfide bond pattern by assisting with the oxidation of cysteine thiols and correcting mis-oxidized substrates. These enhancements can increase the yield of soluble and functionally active protein.