ProtoScript® First Strand cDNA Synthesis Kit features two optimized mixes, M-MuLV Enzyme Mix and M-MuLV Reaction Mix

The kit also contains two optimized primers for reverse transcription and nuclease-free water. An anchored oligo-dT primer [d(T)23VN] forces the primer to anneal to the beginning of the polyA tail. The optimized Random Primer Mix provides random and consistent priming sites covering the entire RNA template including both mRNAs and non-polyadenylated RNAs. The first strand cDNA product generated is more than 10 kb.

M-MuLV Enzyme Mix combines M-MuLV Reverse Transcriptase and Murine RNase Inhibitor, while M-MuLV Reaction Mix contains dNTPs and an optimized buffer.