The NEBNext® dA-Tailing Module has been optimized to efficiently incorporate a non-templated dAMP on the 3´ end of a blunt DNA fragment

3´-dA DNA tailing prevents concatamer formation during subsequent ligation steps. DNA tailed with the NEBNext dA-Tailing module may be ligated to adaptors or cloning vectors with complementary dT overhangs. The NEBNext dA-Tailing Module is provided as a master mix to maximize efficiency and convenience in DNA sample preparation workflows.