The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI

The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. These fragments are best separated by Pulsed Field Gel Electrophoresis.The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size. Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.