Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis

This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 bp on a standard 1% TBE agarose gel.