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Specifications
- Application:
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Methylated DNA
- Sample type:PCR and other enzymatic reactions
- Target molecule:DNA
- Description:EpiMark Bisulfite Conversion Kit
- Size:48 Reactions
- Environmentally Preferable:
- Description:EpiMark Bisulfite Conversion Kit
- Cat. No.:101710-276
- Supplier no.:E3318S
Specifications
About this item
This technique can reveal the methylation status of every cytosine residue, and it is amenable to massively parallel sequencing methods.
Bisulfite conversion involves the conversion of unmodified cytosines to uracil, leaving the modified bases 5-mC and 5-hmC. The EpiMark Bisulfite Conversion Kit is designed for the detection of methylated cytosine, using a series of alternating cycles of thermal denaturation, followed by incubation with sodium bisulfite. This kit includes all the reagents necessary for complete bisulfite conversion, including spin columns. Amplification of bisulfite-treated samples can then be performed using EpiMark Hot Start Taq DNA Polymerase. This kit provides materials for 48 reactions. For optimal results, 50 ng–2 μg DNA is recommended.