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HiPrep SP HP Columns
Catalog # 30621-418
Supplier:  Cytiva
Product available on GSA Advantage®
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HiPrep SP HP Columns
Catalog # 30621-418
Supplier:  Cytiva
Supplier Number:  29-0181-83
Product available on GSA Advantage®

Specifications

  • Flow rate:
    1 - 5 ml/min
  • Particle Size:
    34 µm
  • Max. Pressure:
    0.3 MPa (44 psi)
  • Capacity:
    20 ml
  • Description:
    HiPrep™ SP HP
  • pH Range:
    4 - 13
  • Storage:
    4...30 °C, 20% Ethanol, 0.2 M Sodium Acetate
  • Column hardware:
    PP
  • Bed dimensions:
    16×100 mm
  • Flow velocity:
    <150 cm/h
  • Cat. No.:
    30621-418
  • Supplier no.:
    29018183

Specifications

About this item

HiPrep SP HP 16/10 is a strong cation exchanger designed for preparative ion exchange chromatography separations. This 20 mL column is prepacked with SP Sepharose High Performance chromatography resin. The resin is based on rigid, highly cross-linked beaded agarose with a mean bead diameter of 34 µm for high resolution separations.

  • For high-performance preparative scale separations where high efficiency and high resolution are desired
  • Highly cross-linked agarose provides excellent chemical and physical stability
  • Slim, modern design allows for easy handling and connection

High mechanical strength of the matrix allows for high flow rates, together with outstanding resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.