HiTrap CM FF is prepacked with CM Sepharose Fast Flow, and is a weak cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

• Convenient and affordable for fast, easy ion exchange separations either alone or connected in series
• High flow rates and good scale-up potential
• Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient
• Predictable scale-up

Q, SP, DEAE adn CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.