Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.Two levels of substitution help find optimal selectivity and binding capacity for a given application.

• Easily connected in series to achieve 20 cm bed height
• Small bed volume gives fast results and minimal sample/buffer consumption
• Prepacked, ready-to-use columns for convenient process development optimization
• 10 cm bed height of HiScreen columns is designed to allow method optimization and parameter screening
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

The small column volume of 4.7 ml and bed height of 10 cm make HiScreen columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up. If necessary, two columns can easily be connected in series to give a bed height of 20 cm. Media prepacked in HiScreen HIC columns are also available in other formats and as bulk packs, which makes it possible to use the same media for development work, pilot studies, and routine production.