StrepTactin Sepharose High Performance media for robust, high resolution purification of Strep II-tagged proteins

• Highly pure Strep II-tagged recombinant proteins eluted in concentrated form and small volumes
• Physiological conditions and mild elution preserve target protein activity
• Fast and easy regeneration with 0.5 M NaOH
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• Stable in all commonly used buffers, 0.5 M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents

Mild purification conditions preserve antigenicity and function. This robust and stable Sepharose based medium results in high resolution, sharp concentrated peaks, and reproducible purifications. The binding capacity is also good. Binding capacity is protein dependent. Dynamic binding capacity was tested with GAPDH-Strep(II), Mr 37 400.