Protein A HP SpinTrap™ columns are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids.

• For simple, small-scale antibody purification or enrichment of specific proteins in prepacked, single-use spin columns that are ready to use
• Classic and cross-link protocols provide flexibility
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provide high yield

Prepacked with protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses, easy scale-up with HiTrap™ Protein A HP prepacked columns.

Protein A Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.