PROTEOSTAT® PDI Assay Kit, Enzo Life Sciences

About this item

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity.

The PROTEOSTAT® PDI assay kit is capable of providing a quantitative readout of PDI enzymatic activity in a robust and high-throughput fashion and can be applied to identification of PDI inhibitors from chemical libraries.

This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Delivery: Kit includes PROTEOSTAT® PDI detection reagent, PDI (human) (recombinant), Insulin (Lyophilized), Bacitracin (Inhibitor Control, Lyophilized), PBE buffer, Stop reagent, DTT (10 mM), Deionized water.

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