Biotin-labeled transcripts for microarray internal controls.

Biotin-labeled transcripts prepared from three E. coli genes, bioB, bioC and bioD, and one from bacteriophage P1, cre. These together with the synthetic biotin-labeled deoxyoligonucleotide control (Deoxyoligo B2) are added to biotin-labeled target cRNA before hybridization to probe sequences on the array. These sequences of known concentration provide an internal control for assaying quality and performance. Deoxyoligo B2 aids software programs such as the Affymetrix Microarray Suite by aligning the grid with the array image.