Human Recombinant FasL (soluble)
Supplier: Enzo Life Sciences
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About this item
Fas ligand
Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG®-tag. Glycosylation of rhsFasL is similar to that of natural human FasL.
Specifications
- Protein/Peptide Type:Recombinant
- Species:Human
- Protein/Peptide Name:FasL (soluble)
- Endotoxin Content:<0.1EU/µg purified protein (LAL test; Bio Whittaker).
- Biological Activity:Kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Cross-linking enhancer (see Set Prod. No. ALX-850-014) increases the activity of rhsFasL approx. 50-fold.Attention: Results using sFasL may differ from those obtained with
ED50: 50ng/ml (A20 cells) - Reconstitution Instructions:Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
- Purity:>95% (SDS-PAGE)
- Concentration:0.1mg/ml after reconstitution.
- Formulation:Lyophilized. Contains PBS.
Specifications
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